Journal: Nature chemistry

Article Title: Synthetic O-acetylated sialosides facilitate functional receptor identification for human respiratory viruses.

doi: 10.1038/s41557-021-00655-9

Figure Lengend Snippet: Fig. 4 | Glycan microarray studies reveal binding patterns associated with host specificities. a, Column graphs of viral receptor selectivities. See Supplementary Fig. 9 for the chemical structures of the compounds. Additional results with different concentrations of proteins are presented in Supplementary Fig. 12. Columns show the background-subtracted average relative fluorescence unit (RFU) values of four replicates. Error bars indicate the s.d. of the RFUs. b, Heat-map presentations of viral receptor selectivities. In the heat map, the signal intensities are normalized with the highest value in each protein defined as 1.0 and shown with a colour gradient. Concentrations of Fc-tagged proteins presented are: BCoV S1A 0.3 μg ml–1, OC43 S1A 0.3 μg ml–1, HKU1 S1A 30 μg ml–1, ICV HEF 0.3 μg ml–1, IDV HEF 0.3 μg ml–1, porcine torovirus (PToV) HE 3 μg ml–1, BToV HE 3 μg ml–1, BCoV HE 3 μg ml–1, ECoV HE 3 μg ml–1, RbCoV HE 3 μg ml–1, CRCoV HE 3 μg ml–1, MHV-S HE 3 μg ml–1, ECoV S1A 3 μg ml–1, RbCoV S1A 3 μg ml–1 and CRCoV S1A 10 μg ml–1. Representative surface dissociation constants (Kd,surf) were obtained for the binding of HKU1 S1A with compounds 3g (9-O-Ac, 60 nM) and 3d (7,9-di-O-Ac, 85 nM). See supplementary Fig. 13 for binding curves. Sialoforms 1b and 2b (4,7-di-O-Ac Neu5Ac) were not included in the library because they have not been documented to be naturally occurring.

Article Snippet: The biotinylated compounds were printed on streptavidin-coated glass slides (SuperStreptavidin Microarray Substrate Slides, ArrayIt Inc) using a Scienion sciFLEXARRAYER S3 non-contact microarray equipped with a Scienion PDC80 nozzle (Scienion Inc).

Techniques: Glycoproteomics, Microarray, Binding Assay, Fluorescence

Journal: Biotechnology Reports

Article Title: High-throughput microarray for antimicrobial susceptibility testing

doi: 10.1016/j.btre.2017.10.004

Figure Lengend Snippet: High-throughput AST chip. (A) A schematic of high-throughput antimicrobial susceptibility testing using AST-Chip. Antimicrobial susceptibility testing using the chip can be conducted in 12 h; Microarray micrograph of S. aureus cultures formed on hydrogel spots of AST-Chip after 12–18 h. The spots are interspaced at 1 mm with a spot diameter of 0.5 mm; The nano-biofilms analyzed using FUN-1 is demonstrated in green color; (B) BacLight live/dead assay shows metabolically active cells in yellow-orange color, present as colonies. The scale bar is 100 μm; (C) SEM micrograph of S. aureus nano-biofilm colonies. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Any subsequent dilutions needed for the antimicrobial susceptibility assays were made in PBS, mixed in a suspension of 0.5% alginate, and optimized media, and 50 nL of this mixture was spotted using a non-contact microarray spotter (Omnigrid Micro, Digilab Inc., Holliston, MA) on modified glass slides.

Techniques: High Throughput Screening Assay, Microarray, Live Dead Assay, Metabolic Labelling

Journal: Biotechnology Reports

Article Title: High-throughput microarray for antimicrobial susceptibility testing

doi: 10.1016/j.btre.2017.10.004

Figure Lengend Snippet: Antimicrobial susceptibility profile of S. aureus . AST of methicillin against wild-type (UAMS-1WT) and community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). (A) Microarray scanner image of AST-Chip with S. aureus cultures exposed to different concentrations of methicillin [MET]. The viability is indicated by the fluorescence intensity of the spots. (B) Scanning electron micrographs of S. aureus biofilms that are untreated (top) or treated with 10 μg/ml MET (bottom); (C) Dose-response profile of S. aureus cultures obtained using AST-Chip.

Article Snippet: Any subsequent dilutions needed for the antimicrobial susceptibility assays were made in PBS, mixed in a suspension of 0.5% alginate, and optimized media, and 50 nL of this mixture was spotted using a non-contact microarray spotter (Omnigrid Micro, Digilab Inc., Holliston, MA) on modified glass slides.

Techniques: Microarray, Fluorescence